ABSTRACT
Objective To investigate the effects of volatile oil of Fructus Evodia, mustar d oil and total anthraquinone in Rheum on the transcutaneous permeability of paeoniflorin. Methods Using intelligent transdermal diffusion cell and excised mice skin in vitro as transdermal barrier, the kinetics parameters such as cumulative permeation quantity, permeation rate and permeation lagged time of the three kinds of penetration enhancers on paeoniflorin were determined by HPLC in 12 hours. Results The penetration rate of volatile oil of Fructus Evodia, mustard oil and total anthraquinone in Rheum were 8.188 6, 3.411 7, 1.230 3 μg/(cm2?h), respectively, the enhancement ratios were 22.6, 9.40, 3.40, respectively, and the permeation lagged times were 0.93, 0.51, 0.83 h, respectively. Conclusion Three penetration enhancers all can enhance previously percutaneous absorption of paeoniflorin, which provides reference for the selection of the penetration enhancers of transdermal delivery.
ABSTRACT
Objective To observe the influence of storage time and condition on the chemical components in the volatile oil of Fructus Evodia by GC-MS.Methods The volatile oil was extracted from Fructus Evodia by steam distillation and the amount of each components in the volatile oil was determined by area normalization method and the components were identified by GC-MS.Results One hundred and sixteen components were separated and identified,which accounted for more than 90% in the total constitutes of the volatile oil.The content of volatile oil in Fructus Evodia was influenced by the storage time and condition.Conclusions The method is reliable and has a good repeatability,and can be applied to analyze volatile components in Fructus Evodia.This will provide a certain scientific basis for the storage and clinical application of Fructus Evodia.
ABSTRACT
Objective: A HPCE capillary zone eletrophoretic method for the determination of evodiamine in Fructus Evodia was established. Similarly, areliable HPCE-FPS (fingerprint spectrum) was applied to compare quality of Fructus Evodia to 14 samples of 3 species from different areas. Methods: The evodiamine was detected on 254nm and proteins was on 196nm、254nm by HPCE. The electrophoretic parameters were optimized by studying the efffect of the buffer composition. Results: Calibration graph was constructed in the range 1.944 ?g?mL -1～7.766 ?g?mL -1 for evodiamine (r=0.9974). The regression equation was y= 188.7x-32.1. The average recovery was 101.3 (n=3). In this paper, the proteins of Frutus Evodia HPCE-FPS was established at 196nm and 254nm. Conclusion: Content of evodiamine from Fructus Evodia was determined by HPCE-CZE and HPCE-FPS and distinguished three species from different growing areas related to intrinsic quality of them. The research provides new technique for evaluating quality of traditional Chinese medicine.